Micronutrients, B-Vitamin Panel
What it measures
- FolateLOINC 2284-8
- Vitamin B1 (Thiamine), BloodLOINC 32554-8
- Vitamin B2LOINC 49038-3
- Vitamin B3LOINC 18244-4
- Vitamin B5LOINC 2722-7
- Vitamin B6, PlasmaLOINC 30552-4
- Vitamin B12LOINC 2132-9
Before the visit
Fasting required
Overnight fasting is required. Avoid vitamin supplements for 24 hours prior to collection.
Specimen
- Specimen
- 4 mL serum collected in a red-top tube (no gel) - protected from light and 3 mL frozen plasma collected in an EDTA (lavender-top) tube - protected from light and 2 mL frozen whole blood collected in an EDTA (lavender-top) tube - protected from light
- Minimum volume
- 2 mL serum • 1 mL EDTA plasma • 1 mL EDTA whole blood
- Container
- Amber polypropylene or polyethylene tubes or Neutral color polypropylene or Polyethylene tubes wrapped in aluminum foil to protect from light
- Temperature
- Frozen
Patients should be 18 years of age or greater. Folate & Vitamin B12: Transfer 2 mL (1 mL min) serum into plastic transport tube, ship frozen. Vitamin B2, B3, B5: Transfer 2 mL (1 mL min) serum into an amber vial (or wrap with aluminum foil), ship frozen. Vitamin B6: Transfer 3 mL (1 mL min) EDTA plasma into an amber vial (or wrap with aluminum foil), ship frozen. Separate cells within 24 hours by centrifugation for 5-10 minutes. Transfer plasma to an amber polypropylene or polyethylene transport tube to protect from light. Alternately, neutral color polypropylene or polyethylene tube can be used if wrapped in aluminum foil. Freeze the tubes at -10 to -30° C. Ship frozen. Vitamin B1: Transfer 3 mL (1 mL min) EDTA whole blood into an amber vial (or wrap with aluminum foil), ship frozen. Dietary supplements containing biotin may interfere in assays and may skew analyte results to be either falsely high or falsely low. For patients receiving the recommended daily doses of biotin, draw samples at least 8 hours following the last biotin supplementation. For patients on mega-doses of biotin supplements, draw samples at least 72 hours following the last biotin supplementation. Remove serum or plasma from cells within 4 hours of collection.
Billing
CPT 82746, 84425, 82607, 84252, 84591, 84207