Eosinophil Cationic Protein (ECP)

What it measures

• Eosinophil Cationic Protein (Ecp)LOINC 25638-8

Specimen

Specimen

1 mL serum collected in a red-top tube (no gel)

Minimum volume

0.3 mL

Container

Transport tube

Temperature

Refrigerated (cold packs)

Parameters such as blood collection tube, coagulation time and temperature must be kept within specified limits, since they affect the concentration of released ECP in serum samples. The clotting represents the first incubation in the assay, in which the ECP measured is to be reproducibly released from the eosinophils which have been activated by the inflammation. Collect blood by venipuncture. After collection gently invert the tube 5 times. Release ECP by clotting for 60-120 minutes at room temperature 20-24° C. The temperature must not vary more than + or - 1 degree between sampling occasions to give comparable results. Centrifuge at 1000-1300 x g for 10 minutes at room temperature. Decant serum into a new tube.

Turnaround

Set up: Wed (a.m.)

Billing

CPT 83520